Coding

Part:BBa_K1051802

Designed by: Shihong Chen   Group: iGEM13_Shenzhen_BGIC_ATCG   (2013-09-12)

sgRNA targeting to GFP gene

This part is used to test dCas 9. This part consists of promoter SNR52, sgRNA coding sequence and SUP4 terminator.
Structure of sgRNA is like the picture below

Structure_of_sgRNA.png
This sgRNA can help dCas9(BBa_k1051800) to target to GFP gene to inhibit the expression of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 134
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/asgRNA targeting to GFP gene